所有作者：张雪梅 郭顺堂 李莹辉 宋锦萍
作者单位：中国农业大学食品学院，蛋白质加工利用研究室
论文摘要：Hydrolysates A and P were derived from β-Conglycinin hydrolyzed by Alcalse and Pronase respectively， and then analyzed by gel chromatography of Sephadex G-２５。 Chromatography patterns showed that carbohydrates were mainly existed in fractions A１ of hydrolysate A and P１ of hydrolysate P， with carbohydrate content of ８。２３％ and ９。６６％ respectively， much higher than other fractions。 The results of thin-layer chromatography for fractions A１ and P１ confirmed that the two fractions were carbohydrate-binding peptides， referred as CBP-A and CBP-P respectively。 Two non-carbohydrate peptides A (NCP-A) and P (NCP-P) were derived from CBP-A and CBP-P treated with glycosidase F respectively。 Hydrolysates A and P and fractions from them were observed inhibitory effect on mitogen-induced proliferation of murine splenocytes。 Compared to NCP-A and A２， CBP-A had lower inhibitory effect at concentration of ０。５~５μg/ml， but stronger inhibitory effect at concentration of ５００~１００μg/ml。 CBP-P had stronger inhibitory effect on mitogen-induced proliferation of splenocytes at all concentration of ０。５~１０００μg/ml except at ５０μg/ml。 These results indicated that glycopeptides could be isolated from soy peptides。 And inhibitory effects on mitogen-induced proliferation of splenocytes were influenced by carbohydrate chain。
关键词： β-伴大豆球蛋白 糖肽 增殖 脾细胞
免费下载《β-伴大豆球蛋白酶解物中分离鉴定糖肽及其对小鼠脾细胞增殖效果的研究》PDF全文（已停止下载）
本站“论文下载”文章收集整理于“中国科技论文在线”，由于各种原因，本站已暂停论文下载！请前往“中国科技论文在线http://www.paper.edu.cn/”免费下载！
指出，范文大全立刻进行改正或删除有关内容! 
当前位置：