所有作者：陈国珍 田杰 朱静 孙慧超 吕铁伟 吴刚
作者单位：重庆医科大学附属儿童医院
论文摘要：Objective： To study the process of mouse heart development and the spatiotemporal expression pattern of histone acetyltransferase steroid receptor coactivator-１ (SRC１) in developmental hearts of mice． Methods： The normal mouse hearts from E７。５~E１８ and neonatal １ day as well as ３ months were included in this study。 Histological technique was used to observe the morphology of developmental hearts。 Immunohistochemistry and Western blot techniques were performed to examine the temporal and spatial expression of SRC１ during cardiogenesis in mice。 Results： １。 The heart was initiated in crescent-shaped cardiac primordium at about mouse embryo day ７。５ (E７。５)。 Soon after that， the cardiac crescent converged to form the primary linear cardiac tube at about E８。５ and looping cardiac tube at E９。５。 At E１０。５， atrioventricular canal formed as well as atrioventricular endocardial cushion (EC) and protrusive trabeculae had been visible。 Interatrial septum formed at E１１。５～E１３。５ and interventricular septum formed at E１１。５～E１４。５ and atrioventricular valve formed at E１２。５～E１５。５ and outflow tract was septated into pulmonary artery and aorta at E１１。５~E１３。５。 Cardiomyocyte differentiation and multiplication made the walls compact and thicken at E１１。５～E１６。５ while cardiomyocyte multiplication and increase caused cardiac walls to become thicker and the heart to become bigger at E１６。５～postnatal day １。 During postnatal stage， with the growth of the mouse， heart became big yet cardiomyocyte no multiplication longer。 ２。 No expression of SRC１ was detected in heart primordium at E７。５； SRC１ was found very weak expression in cardiac tube at E８。５~E９。５； SRC１ was found weak expression in trabeculae and relatively strong and widespread expression in other heart regions after E１０。５。 In the developmental hearts after E１０。５， SRC１ proteins relatively lowly expressed at E１０。５ and reached the expression peak at E１１。５~E１２。５ and gradually decreasingly expressed in the subsequent developmental stages。 Conclusion： The heart development in mice is similar to human’s。 It undergoes cardiac progenitor cells induction and specification， heart tube formation and looping， chamber specification and growth and later developmental completion to form four-chambered heart。 There were widespread distributions and dynamic expression variants of SRC１ during mouse heart development， hinting it was related to the regulation of the heart development。
关键词： histone acetyltransferases SRC1 heart development cardiogenesis
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