Shifts in microbial community under different fertilizers in a lab incubation

论文发布时间：[2010-01-05] 范文大全编辑：Voive.net

所有作者：张奇春王光火

作者单位：浙江大学

论文摘要：The effects of different fertilization treatments on paddy soil microbial communities were characterized using several techniques。 Treatments included no fertilizer (NF)， inorganic fertilizer only (CF)， pig manure in combination with inorganic fertilizer (CFM) and straw manure in combination with inorganic fertilizer (CFS)。 Assessments included determination of microbial population counts by soil dilution plating on various general and selective culture media， microbial activity by community level physiological profiling using Biolog sole carbon source utilization tests (Biolog) and phospholipid fatty acid (PLFA) profiles。 Soil populations with organic manure tended to have higher levels of culturable bacteria and fungi and lower levels of actinomycetes。 Biolog plates indicated that the soil microbial metabolism quotient (AWCD)， Shannon index and McIntonsh index all increased significantly in CFS- and CFM-treated soils relative to NF-treated soil。 Soil phospholipid fatty acid (PLFA) analysis demonstrated the monounsaturated fatty acids １８：１ω９c，１８：１ω７c，１６：１ω７c，１６：１ω９c，１５：１ω６c and １８：２ω６，９c were most prevalent in CFM- and CFS-treated soils。 The saturated straight chain lipids １４：０，１７：０，１８：０，１９：０ and ２０：０ were most enriched in NF- and CF-treated soils。 Fertilization increased the PLFA biomarkers for bacteria and fungi， but reduced the markers for actinomycetes。 The ratio of Gram-positive to Gram-negative bacteria was significantly higher in the NF- and CF-treated soils relative to the CFS- and CFM-treated soils (P

关键词： Paddy soil Fertilization Soil microbial community Biolog Phospholipid fatty acid (PLFA)

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